Journal: Life sciences

Article Title: Calcimimetic R568 inhibits tetrodotoxin-sensitive colonic electrolyte secretion and reduces c-fos expression in myenteric neurons

doi: 10.1016/j.lfs.2017.12.019

Figure Lengend Snippet: A: DAPI staining of the nuclei. B: c-fos staining. C: β-tubulin staining. D: merge of A, B and C. All c-fos protein co-expressed with DAPI and was restricted within myenteric ganglion areas. Arrow with dotted line indicates lack of expression of c-fos in a DAPI-stained nucleus. Arrow with solid line indicates co-expression of c-fos and DAPI in a nucleus of neuron. Scale Bars = 10μm

Article Snippet: After three 10 min washes in 0.1 M PBS, tissues were incubated with Alexa Fluor 488 conjugated goat anti-rabbit IgG (H+L) F(ab′) 2 fragment (1:800, Cell Signaling Technology, Beverly, MA, USA) for 1 h, washed 10 min in 0.1 M PBS ×3, and the sections were mounted with DAPI Fluoromount-GTM (Citifluor, UK).

Techniques: Staining, Expressing